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KMID : 0380620080400060696
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Korean Journal of Food Science and Technology
2008 Volume.40 No. 6 p.696 ~ p.701
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Cytoprotective Effect by Antioxidant Activity of Codonopsis lanceolata and Platycodon grandiflorum Ethyl Acetate Fraction in Human HepG2 Cells
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Kim Soo-Hyun
Choi Hyun-Jin
Oh Hyun-Taek
Chung Mi-Ja
Cui Cheng-Bi
Ham Seung-Shi
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Abstract
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The objective of this study was to determine whether Codonopsis lanceolata or Platycodon grandiflorum ethylacetate fraction (CLEA or PGEA) protect cells against sodium nitroprusside (SNP)-induced oxidative stress via theexpression of various antioxidant systems. The HepG2 cells exposed for 24hr to 0.5mM SNP showed a reduction in thecell viability by an MTT assay. Pretreatment with CLEA and PGEA resulted in an inhibition of SNP-induced cell death.In addition, the effects of CLEA and PGEA on the expression of antioxidant systems via RT-PCR analyses was assessed.The levels of catalase (CAT), glucose-6-phosphate dehydrogenase (G6PD) and metallothionein (MT)-1A mRNA wereincreased after 24 hr of CLEA exposure. The levels of Mn superoxide dismutase CAT, G6PD, MT-1A, and MT-2A mRNAwere increased after PGEA treatment. In conclusion, CLEA and PGEA exert indirect antioxidant effects, perhaps via theinduction of a variety of antioxidant systems which, may protect cells against oxidative stress.
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KEYWORD
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Codonopsis lanceolata, Platycodon grandiflorum, antioxidant, oxidative stress, antioxidant systems
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